University of Pittsburgh Department of Cell Biology
  • Research

    The central theme of my research program is to dissect molecular signaling pathways controlling vascular function in rodent and zebrafish model systems of disease. This work encompasses the development and validation of the system-based microscopic approaches to collect real time metrics of vascular performance (blood flow, vascular reactivity and barrier function) in transgenic zebrafish models.  This in vivo systems-based approach allows us to observe endothelial function/dysfunction at the molecular level and provide: 1) mechanistic insight into diverse disease processes; 2) a potential way to identify key target molecules for future prophylactic and/or treatment strategies; 3) avenues for the testing of potential therapeutic interventions. This model system and my imaging approaches are central to funded collaborations with colleagues at Carnegie Mellon University to develop novel genetically encoded fluorescent biosensors for reactive oxygen and nitrogen species (ROS and RNS, respectively), and to apply these new sensors to establish the role of ROS and RNS based signaling in the regulation of vascular tone and barrier function in cell culture and animal models of vascular disease.

  • Publications

    1. Kagan VE, Mao G, Qu F, Angeli JP, Doll S, Croix CS, Dar HH, Liu B, Tyurin VA, Ritov VB, Kapralov AA, Amoscato AA, Jiang J, Anthonymuthu T, Mohammadyani D, Yang Q, Proneth B, Klein-Seetharaman J, Watkins S, Bahar I, Greenberger J, Mallampalli RK, Stockwell BR, Tyurina YY, Conrad M, Bayır H. Oxidized arachidonic and adrenic PEs navigate cells to ferroptosis.. Nat Chem Biol. 2017 Jan;13(1):81-90. doi: 10.1038/nchembio.2238. PubMed PMID: 27842066;
    2. St Croix CM, Steinhorn RH. New Thoughts about the Origin of Plexiform Lesions.. Am J Respir Crit Care Med. 2016 Mar 1;193(5):484-5. doi: 10.1164/rccm.201510-1959ED. PubMed PMID: 26930432;
    3. He J, Wang Y, Missinato MA, Onuoha E, Perkins LA, Watkins SC, St Croix CM, Tsang M, Bruchez MP. A genetically targetable near-infrared photosensitizer.. Nat Methods. 2016 Mar;13(3):263-8. doi: 10.1038/nmeth.3735. PubMed PMID: 26808669;
    4. Phinney DG, Di Giuseppe M, Njah J, Sala E, Shiva S, St Croix CM, Stolz DB, Watkins SC, Di YP, Leikauf GD, Kolls J, Riches DW, Deiuliis G, Kaminski N, Boregowda SV, McKenna DH, Ortiz LA. Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs.. Nat Commun. 2015 Oct 7;6():8472. doi: 10.1038/ncomms9472. PubMed PMID: 26442449;
    5. Watkins SC, Maniar S, Mosher M, Roman BL, Tsang M, St Croix CM. High resolution imaging of vascular function in zebrafish.. PLoS One. 2012;7(8):e44018. doi: 10.1371/journal.pone.0044018. PubMed PMID: 22952858;
    6. Garner AL, St Croix CM, Pitt BR, Leikauf GD, Ando S, Koide K. Specific fluorogenic probes for ozone in biological and atmospheric samples.. Nat Chem. 2009 Jul;1(4):316-21. doi: 10.1038/nchem.240. PubMed PMID: 20634904;
    7. Bernal PJ, Leelavanichkul K, Bauer E, Cao R, Wilson A, Wasserloos KJ, Watkins SC, Pitt BR, St Croix CM. Nitric-oxide-mediated zinc release contributes to hypoxic regulation of pulmonary vascular tone.. Circ Res. 2008 Jun 20;102(12):1575-83. doi: 10.1161/CIRCRESAHA.108.171264. PubMed PMID: 18483408;

     

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