University of Pittsburgh Department of Cell Biology

  • Research

    Heart development as a critical embryonic developmental process is tightly regulated on the cellular and molecular level. If this process goes awry, it will lead to congenital heart diseases (CHD), which accounts for a significant portion of stillbirths and is present in 1-2% of all live births. The Li lab is using advanced techniques including human induced pluripotent stem cells (hiPSC), single-cell RNA sequencing, single molecular in situ hybridization, CRISPR/Cas9, and tissue cleaning methods to decode the spatial and temporal information of each single cardiac cells. Meanwhile, with the knowledge gained from the study of basic cardiac lineage regulations, the lab is also exploring the lineage defects in congenital heart diseases using patient-derived iPSCs.             

  • Publications

    1. Feng W, Przysinda A, Li G. Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells.. J Vis Exp. 2020 Jan 7;(155):. doi: 10.3791/60647. PubMed PMID: 31984954;
    2. Feng W, Chen L, Nguyen PK, Wu SM, Li G. Single Cell Analysis of Endothelial Cells Identified Organ-Specific Molecular Signatures and Heart-Specific Cell Populations and Molecular Features.. Front Cardiovasc Med. 2019;6():165. doi: 10.3389/fcvm.2019.00165. PubMed PMID: 31850371;
    3. Li G, Tian L, Goodyer W, Kort EJ, Buikema JW, Xu A, Wu JC, Jovinge S, Wu SM. Single cell expression analysis reveals anatomical and cell cycle-dependent transcriptional shifts during heart development.. Development. 2019 Jun 14;146(12):. doi: 10.1242/dev.173476. PubMed PMID: 31142541;

     

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