|
Raymond A. Frizzell, Ph.D.. |
Our lab is interested in the mechanisms of chloride and sodium transport across absorptive and secretory epithelial cells of the lung and kidney, in the biogenesis and quality control pathways that influence ion channel expression, and in the membrane trafficking mechanisms that govern channel density at the cell surface. This work focuses primarily on the anion channel, CFTR, whose mutation produces the genetic disease, cystic fibrosis, and on the epithelial sodium channel, ENaC, a determinant of airway surface liquid volume and blood pressure regulation.
Carol A. Bertrand, Ph.D.
Michael B. Butterworth, Ph.D.
Kathryn W. Peters, Ph.D.
Bela Z. Schmidt, Ph.D.
Fei Sun, Ph.D.
- Ahner A, Nakatsukasa K, Zhang H, Frizzell RA, Brodsky JL. Small heat-shock proteins select deltaF508-CFTR for endoplasmic reticulum-associated degradation. Mol Biol Cell. 2007 18(3):806-14.
- Butterworth MB, Edinger RS, Ovaa H, Burg D, Johnson JP, Frizzell RA. The deubiquitinating enzyme, UCH-L3 regulates the apical membrane recycling of the epithelial sodium channel (ENaC). J Biol Chem. 2007 Oct 29; [Epub ahead of print]
- Hill WG, Butterworth MB, Wang H, Edinger RS, Lebowitz J, Peters KW, Frizzell RA, Johnson JP. The epithelial sodium channel (ENaC) traffics to apical membrane in lipid rafts in mouse cortical collecting duct cells. J Biol Chem. 2007 Oct 10; [Epub ahead of print]
- Bertrand CA, Laboisse C, Hopfer U, Bridges RJ, Frizzell RA. Methods for detecting internalized, FM 1-43 stained particles in epithelial cells and monolayers. Biophys J. 2006 91(10):3872-83.
- Liang X, Peters KW, Butterworth MB, Frizzell RA. 14-3-3 isoforms are induced by aldosterone and participate in its regulation of epithelial sodium channels. J Biol Chem. 2006 281(24):16323-32.
- Myerburg MM, Butterworth MB, McKenna EE, Peters KW, Frizzell RA, Kleyman TR, Pilewski JM. Airway surface liquid volume regulates ENaC by altering the serine protease-protease inhibitor balance: a mechanism for sodium hyperabsorption in cystic fibrosis. J Biol Chem. 2006 281(38):27942-9.
- Sun F, Zhang R, Gong X, Geng X, Drain PF, Frizzell RA. Derlin-1 promotes the efficient degradation of the cystic fibrosis transmembrane conductance regulator (CFTR) and CFTR folding mutants. J Biol Chem. 2006 281(48):36856-63.
- Wang H, Traub LM, Weixel KM, Hawryluk MJ, Shah N, Edinger RS, Perry CJ, Kester L, Butterworth MB, Peters KW, Kleyman TR, Frizzell RA, Johnson JP. Clathrin-mediated endocytosis of the epithelial sodium channel. Role of epsin. J Biol Chem. 2006 281(20):14129-35.
- Zhang H, Schmidt BZ, Sun F, Condliffe SB, Butterworth MB, Youker RT, Brodsky JL, Aridor M, Frizzell RA. Cysteine string protein monitors late steps in cystic fibrosis transmembrane conductance regulator biogenesis. J Biol Chem. 2006 281(16):11312-21.
- Butterworth MB, Edinger RS, Johnson JP, Frizzell RA. Acute ENaC stimulation by cAMP in a kidney cell line is mediated by exocytic insertion from a recycling channel pool. J Gen Physiol. 2005 125(1):81-101.
- Bertrand CA, Frizzell RA. The role of regulated CFTR trafficking in epithelial secretion. Am J Physiol Cell Physiol. 2003 285(1):C1-18. Review.