University of Pittsburgh Department of Cell Biology
    • Natalia V. Varlakhanova, Ph.D.

    Natalia V. Varlakhanova, Ph.D.

    Research Assistant Professor
    Tel: 412-383-9026
    Fax: 412-648-8330
    Address: S355 BST-South
    nvv@pitt.edu

  • Research

    I am interested in the molecular mechanisms of endosomal trafficking and membrane remodeling, and how these highly complex processes are integrated into the TORC1 signaling network. The primary model I utilize is the budding yeast Saccharomyces cerevisiae. Membrane remodeling processes are integral to endosomal morphology, function in cargo sorting and trafficking. I focus on a set of proteins involved in membrane remodeling. These include the dynamin-related proteins, the sorting nexins and retromer. I combine structural approaches with live-cell imaging and classical yeast genetics and molecular biology. Recently, membrane remodeling has also emerged as a key regulator of signaling by TORC1, the master controller of cell growth decisions in all eukaryotic cells. Using high-throughput genetics, in-vivo imaging and biochemistry, I am dissecting the signaling pathways regulating TORC1 rEsponses to starvation.

  • Publications

    1. Varlakhanova NV, Tornabene BA, Ford MGJ. Feedback regulation of TORC1 by its downstream effectors Npr1 and Par32.. Mol Biol Cell. 2018 Nov 1;29(22):2751-2765. doi: 10.1091/mbc.E18-03-0158. PubMed PMID: 30156471;
    2. Varlakhanova NV, Tornabene BA, Ford MGJ. Ivy1 is a negative regulator of Gtr-dependent TORC1 activation.. J Cell Sci. 2018 Sep 7;131(17):. pii: jcs218305. doi: 10.1242/jcs.218305. PubMed PMID: 30097557;
    3. Varlakhanova NV, Alvarez FJD, Brady TM, Tornabene BA, Hosford CJ, Chappie JS, Zhang P, Ford MGJ. Structures of the fungal dynamin-related protein Vps1 reveal a unique, open helical architecture.. J Cell Biol. 2018 Oct 1;217(10):3608-3624. doi: 10.1083/jcb.201712021. PubMed PMID: 30087125;
    4. Varlakhanova NV, Mihalevic MJ, Bernstein KA, Ford MGJ. Pib2 and the EGO complex are both required for activation of TORC1.. J Cell Sci. 2017 Nov 15;130(22):3878-3890. doi: 10.1242/jcs.207910. PubMed PMID: 28993463;

     

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