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Linton
M. Traub, PhD
Assistant Professor
Tel: 412-648-9711
Fax: 412-648-8330
traub@pitt.edu
PubMed
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 Many
molecules enter the cell interior within clathrin-coated
vesicles, in process termed endocytosis. This process
is critical to the way we move and think. At the tip of
each axon, synaptic vesicles (packages of neurotransmitter)
release their contents when the nerve is stimulated by
fusing with the cell surface. Almost instantly, the membrane
of the synaptic vesicle is then retrieved within clathrin-coated
vesicles. Endocytosis is thus tightly coupled to exocytosis,
the stimulated release of neurotransmitter. Failure to
recover synaptic-vesicle membrane results in both morphological
disruption of the nerve terminal and defective neurotransmission.
We study the mechanisms and molecules involved in clathrin-coat
assembly. To understand how these complex structures assemble
within only a minute or two, we use biochemical, cell
biological and structural approaches to unravel the protein-protein
interactions that orchestrate the formation of this elaborate
protein-sorting machine.
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Mishra, S.K., P. A. Keyel, M. J. Hawryluk, N.R. Agostinelli, S. C. Watkins and L.M. Traub. Disabled-2 exhibits the properties of a cargo-selective endocytic clathrin adaptor. EMBO J. 21: 4915-4926, 2002.
- Mishra, S.K., S.C. Watkins and L.M. Traub. The autosomal recessive hypercholesterolemia (ARH) protein interfaces directly with the clathrin-coat machinery. Proc. Natl. Acad. Sci. U S A. 99:16099-16104, 2002.
- Bonifacino, J.S., and L.M. Traub. Signals for sorting of transmembrane proteins to endosomes and lysosomes. Annu. Rev. Biochem. 72: 395-447, 2003.
- Traub, L.M. Sorting it out: AP-2 and alternate clathrin adaptors in endocytic cargo selection. J. Cell Biol. 163: 203-208, 2003
- Miele, A.E., P.J. Watson, P.R. Evans, L.M. Traub and D.J. Owen. Two distinct interaction motifs in amphiphysin bind two independent sites on the clathrin terminal domain β-propeller. Nat. Struc. & Mol. Biol. 11: 242-248, 2004.
- Mishra, S.K., P.A. Keyel, M.A. Edeling, A.L. Dupin, D.J. Owen and L.M. Traub. Functional dissection of an AP-2 β2 appendage-binding sequence within the autosomal recessive hypercholesterolemia (ARH) protein. J. Biol. Chem. 280: 19270-19280, 2005
- Hawryluk, M.J., P.A. Keyel, S.K. Mishra, S.C. Watkins, J.E. Heuser, and L.M. Traub. Epsin 1 is a polyubiquitin-selective clathrin-associated sorting protein. Traffic 7: 262-281, 2006.
- Edeling, M.A., S.K. Mishra, P.A. Keyel, A.L. Steinhauser, B.M. Collins, R. Roth, J.E. Heuser, D.J. Owen, and L.M. Traub. Molecular switches Involving the AP-2 β2 appendage regulate endocytic cargo selection and clathrin coat assembly. Dev. Cell 10: 329-342, 2006.
- Keyel, P.A., S.K. Mishra, R. Roth, J.E. Heuser, S.C. Watkins, and L.M. Traub. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors. Mol. Biol. Cell 17: 4300-4317, 2006.
- Brett, T.J. and L.M. Traub. Molecular structures of coat and coat-associated proteins: function follows form. Curr. Opin. Cell Biol. 18: 395-406, 2006.
- Traub, L.M. and Lukacs, G.L. Decoding ubiquitin sorting signals for clathrin-dependent endocytosis by CLASPs. J. Cell Sci. 120: 543-553, 2007.
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