University of Pittsburgh Department of Cell Biology
    • Kathryn W. Peters, Ph.D.

    Kathryn W. Peters, Ph.D.

    Research Assistant Professor
    Tel: 412-692-9336
    Fax: 412-692-8906
    Address: 7161 Rangos Research Center, Children's Hospital of Pittsburgh
    kathi@pitt.edu
  • Research

    Numerous functions have been ascribed to the cystic fibrosis transmembrane conductance regulator (CFTR) and all of them rely on the proper trafficking of this protein into the apical plasma membrane. Our understanding is based on the finding that the most common mutation, Δ-F508 CFTR, remains in the endoplasmic reticulum and is degraded. In contrast, drug or temperature rescue allows mutant Δ-F508 CFTR to become fully glycosylated and traffic to the membrane. We will assess the impact of other treatments on mutant CFTR by making use of lipid rafts in which we observe migration of mature CFTR, endogenous or corrected, to a lower buoyant density in a sucrose gradient. In addition, we will create and evaluate antibodies that detect corrected Δ-F508 at the cell surface. We will identify accessory proteins that participate in this process so that we can gain insight into therapies that can be targeted to advance CFTR from within the cell to its functional location.

  • Publications

    1. Xiubin Liang, Michael B. Butterworth, Kathryn W. Peters, William H. Walker, and Raymond A. Frizzell (2008). An obligatory heterodimer of 14-3-3Β and 14-3-3ε is required for aldosterone regulation of the epithelial sodium channel. J. Biol. Chem. 283: 27418-27425
    2. Christopher M. Lewarchik, Kathryn W. Peters, Juanjuan Qi, and Raymond A. Frizzell (2008). Regulation of CFTR trafficking by its R domain. J. Biol. Chem. 283: 28401-28412.
    3. Moser, A. James, A. Gangopadhyay, N. A. Bradbury, K. W. Peters, R. A. Frizzell, R. J. Bridges (2007). Electrogenic bicarbonate secretion by Prairie Dog Gallbladder. Mar 15 epub ahead of print. Am. J. Physiol.
    4. Hill, Warren G., Michael B. Butterworth, Huamin Wang, Robert S. Edinger, Jonathan Lebowitz, Kathryn W. Peters, Raymond A. Frizzell, and John P. Johnson (2007). The epithelial sodium channel (ENaC) traffics to apical membrane in lipid rafts in mouse cortical collecting duct cells. J. Biol. Chem. 282: 37402-37411.
    5. Kreindler, James L., Kathryn W. Peters, Raymond A. Frizzell, Robert J. Bridges (2006). Identification and membrane localization of electrogenic sodium bicarbonate cotransporters in Calu- 3 cells. Biochim. Biophys. Acta. 1762: 704-710.
    6. Liang, X., K.W. Peters, M.B. Butterworth, and R.A. Frizzell (2006). 14-3-3 isoforms are induced by aldosterone and participate in its regulation of epithelial sodium channels. J Biol. Chem. 281: 16323-16332.
    7. Myerburg, Mike. M., Michael B. Butterworth, Erin E. McKenna, Kathryn W. Peters, Raymond A. Frizzell, Thomas R. Kleyman, and Joseph M. Pilewski (2006). Airway surface liquid volume regulates ENaC by altering the serine protease-protease inhibitor balance: a mechanism for sodium hyperabsorption in cystic fibrosis. J. Biol. Chem. 281: 27942-27949.
    8. Wang, H., L. M. Traub, K. M. Weixel, M. J. Hawryluk, N. Shah, R. S. Edinger, C. J. Perry, L. Kester, M. B. Butterworth, K. W. Peters, T. R. Kleyman, R. A. Frizzell, J. P. Johnson (2006). Clathrin-mediated endocytosis of the epithelial sodium channel. Role of epsin. J. Biol. Chem. 281: 4129-14135.

     

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