University of Pittsburgh Department of Cell Biology
    • Sanjay Mishra, PhD

    Sanjay Mishra, PhD

    Research Assistant Professor
    Tel: 412-692-9334
    Fax: 412-692-8906
    Address: 7161 Rangos Research Center, Children's Hospital of Pittsburgh
    skm25@pitt.edu
  • Research

    Function of a newly synthesized protein depends on their three-dimensional confirmations. The cells utilize its evolutionary conserved machinery to maintain the protein quality. Protein quality control plays an important role at the cellular level in the removal of misfolded and aggregated proteins. The folding mechanism is mainly assisted by molecular chaperones, which are generally heat shock proteins. Dysregulation of these mechanism leads to the pathogenesis of number of diseases, such as cystic fibrosis and neurodegenerative disease. Cystic fibrosis transmembrane conductance regulator (CFTR) is one of the challenged proteins with number of misfolding defects, which leads to the variety of severity in the patients. Approximately 2000 mutations evidenced in the CF gene, out of which ~120 are recognized to be CF causing mutations. Our aim is to elucidate the proper folding and trafficking of CFTR to the apical membrane of the epithelial cells.

  • Publications

    1. Mishra SK, Funair L, Cressley A, Gittes GK, Burns RC. High-affinity Dkk1 receptor Kremen1 is internalized by clathrin-mediated endocytosis. PLoS One. 2012;7(12):e52190. doi: 10.1371/journal.pone.0052190. Epub 2012 Dec 14. [link]
    2. Chen H, Houshmand G, Mishra S, Fong GH, Gittes GK, Esni F. Impaired pancreatic development in Hif2-alpha deficient mice. Biochem Biophys Res Commun. 2010 Aug 27;399(3):440-5. doi: 10.1016/j.bbrc.2010.07.111. Epub 2010 Aug 3. [link]
    3. Reider A, Barker SL, Mishra SK, Im YJ, Maldonado-Báez L, Hurley JH, Traub LM, Wendland B. Syp1 is a conserved endocytic adaptor that contains domains involved in cargo selection and membrane tubulation. EMBO J. 2009 Oct 21;28(20):3103-16. doi: 10.1038/emboj.2009.248. Epub 2009 Aug 27. [link]
    4. Thieman JR, Mishra SK, Ling K, Doray B, Anderson RA, Traub LM. Clathrin regulates the association of PIPKIgamma661 with the AP-2 adaptor beta2 appendage. J Biol Chem. 2009 May 15;284(20):13924-39. doi: 10.1074/jbc.M901017200. Epub 2009 Mar 14. [link]
    5. Mishra SK, Jha A, Steinhauser AL, Kokoza VA, Washabaugh CH, Raikhel AS, Foster WA, Traub LM. Internalization of LDL-receptor superfamily yolk-protein receptors during mosquito oogenesis involves transcriptional regulation of PTB-domain adaptors. J Cell Sci. 2008 Apr 15;121(Pt 8):1264-74. doi: 10.1242/jcs.025833. [link]
    6. Keyel PA, Mishra SK, Roth R, Heuser JE, Watkins SC, Traub LM. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors. Mol Biol Cell. 2006 Oct;17(10):4300-17. Epub 2006 Jul 26. [link]
    7. Edeling MA, Mishra SK, Keyel PA, Steinhauser AL, Collins BM, Roth R, Heuser JE, Owen DJ, Traub LM. Molecular switches involving the AP-2 beta2 appendage regulate endocytic cargo selection and clathrin coat assembly. Dev Cell. 2006 Mar;10(3):329-42. [link]
    8. Hawryluk MJ, Keyel PA, Mishra SK, Watkins SC, Heuser JE, Traub LM. Epsin 1 is a polyubiquitin-selective clathrin-associated sorting protein. Traffic. 2006 Mar;7(3):262-81. Erratum in: Traffic. 2006 Jul;7(7):927. [link]
    9. Mishra SK, Keyel PA, Edeling MA, Dupin AL, Owen DJ, Traub LM. Functional dissection of an AP-2 beta2 appendage-binding sequence within the autosomal recessive hypercholesterolemia protein. J Biol Chem. 2005 May 13;280(19):19270-80. Epub 2005 Feb 22. [link]

     

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