Physical Molecular Biology Section
University of Pittsburgh Laboratory of Physical Molecular Biology; Sanford Leuba, Ph.D.
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Chromatin Fibers Compact with Salt
   
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0.1 mM MgCl

0.1 mM MgCl

0.2 mM MgCl
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0.2 mM MgCl

0.3 mM MgCl

0.3 mM MgCl
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0.4 mM MgCl

0.4 mM MgCl

0.5 mM MgCl
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0.5 mM MgCl
MgCl-induced compaction of
the extended chromatin fiber,
as visualized by SFM imaging.
Chromatin erythrocyte fibers,
glutaraldehyde-fixed at the
respective salt concentrations
were dialyzed versus 5 mM
triethanolamine-HCl pH 7.0
and deposited on freshly
cleaved mica from these
solutions. Imaging is in air.
Salt concentrations are marked
below each panel. Images were
taken in collaboration with
Dr. G. Yang.
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Zlatanova, J., Leuba, S. H., and van Holde, K. Chromatin fiber structure: morphology, molecular determinants, structural transitions. Biophys. J. 74(5) 2554-2566 1998 PDF file