Cellular and molecular characteristics of epithelial Na+ channels. Recent work has focused on the defining the subunit stoichiometry of the Na+ channel, characterizing the structure of the channel’s pore, and on the identification of sites within Na+ channel subunits that facilitate subunit interactions and assembly.
Regulation of epithelial Na+ channels. We are examining mechanisms by which selected hormones regulate epithelial Na+ channels. Recent studies have focused on the regulation of epithelial Na+ channels by mechanical forces and by the hormone aldosterone. We have initiated studies directed at identifying K+ channels in renal collecting tubules that are regulated by mechanical forces.
Functional interactions between epithelial Na+ channels and cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channels. We are examining mechanisms by which Na+ channels are functionally altered in cystic fibrosis.
Publications
Suaud, L., J. Li, Q. Jiang, R.C. Rubenstein, and T.R. Kleyman. Genistein restores functional interactions between DF508-CFTR and ENaC in Xenopus oocytes. J. Biol. Chem. 277: 8928-8933, 2002.
Kleyman, T.R., J.B. Zuckerman, P. Middleton, K.A. McNulty, B. Hu, X. Su, B. An, D.C. Eaton, and P.R. Smith. Cell surface expression and turnover of the alpha subunit of the epithelial sodium channel. Am. J. Physiol. 281: 213-221, 2001
Satlin, L.M., S. Sheng, C. Woda, and T.R. Kleyman. Epithelial Na+ channels are regulated by flow. Am. J. Physiol. 280: F1010-F1018, 2001
Sheng, S., J. Li, K.A. McNulty, T. Kieber-Emmons, and T.R. Kleyman. Epithelial sodium channel pore region: Structure and role in gating. J. Biol. Chem. 276:1326-1334, 2001.
Sheng, S., K.A. McNulty, J.M. Harvey, and T.R. Kleyman. Second Transmembrane Domains of ENaC Subunits Contribute to Ion Permeation and Selectivity. J. Biol. Chem. 276:44091-44098, 2001.
