University of Pittsburgh Department of Cell Biology
  • Research

    The primary research interests of the lab focus on the regulation of airway surface liquid (ASL) pH and mucin secretion in epithelia, and the involvement of ion channels in modulating the process. Both bicarbonate and mucin contribute to the pH of the ASL, which varies considerably in disease from acidic in CF to alkaline in chronic bronchitis. Current work centers on the biosynthesis and activity of chloride channels and anion exchangers that complement and may regulate the CFTR chloride channel, as well as the apical membrane permeability to bicarbonate. In addition, ongoing effort is devoted towards the development and refinement of methods for performing electrophysiology and live cell fluorescence microscopy.

  • Publications

    1. Silvis MR, Bertrand CA, Ameen N, Golin-Bisello F, Butterworth MB, Frizzell RA, Bradbury NA. Rab 11b regulates the apical recycling of CFTR in polarized intestinal epithelial cells. Mol Biol Cell (2009); 20:2337-2350.
    2. Bertrand CA, Zhang R, Pilewski JM, Frizzell RA. SLC26A9 is a constitutively active, CFTR-regulated anion conductance in human bronchial epithelia. J Gen Physiol (2009); 133(4):421-438.
    3. Kreindler JL, Bertrand CA, Lee RJ, Karasic T, Aujla S, Pilewski JM, Frizzell RA, Kolls JK. Interleukin-17A induces bicarbonate secretion in normal human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol (2009); 296(2):L257-66.
    4. Sun F, Mi Z, Condliffe SB, Bertrand CA, Gong X, Lu X, Zhang R, Latoche JD, Pilewski JM, Robbins PD, Frizzell RA. Chaperone displacement from mutant cystic fibrosis transmembrane conductance regulator restores its function in human airway epithelia. FASEB J (2008); 22(9):3255-63.
    5. Bertrand CA, Laboisse C, Hopfer U, Bridges RJ, and Frizzell RA. (2006) Methods for detecting internalized, FM 1-43 stained particles in epithelial cells and monolayers. Biophys J.; 91:3872-3883.
    6. Bertrand CA, Danahay H, Poll CT, Laboisse C, Hopfer U, Bridges RJ. Niflumic acid inhibits ATP-stimulated exocytosis in a mucin-secreting epithelial cell line. Am J Physiol (2004); 286:C247-C255.
    7. Silvis MA, Picciano JA, Bertrand CA, Weixel KM, Bridges RJ, Bradbury NA. A mutation in the cystic fibrosis transmembrane conductance regulator generates a novel internalization sequence and enhances endocytic rates. J Biol Chem (2003); 278:11554-11560.
    8. Bertrand, CA and RA Frizzell. The role of regulated CFTR trafficking in epithelial secretion. Am J Phys (2003); 285:C1-C18.
    9. Bertrand, C.A. and U. Hopfer. (2002). Measurement of Membrane Capacitance in Epithelial Monolayers. In: Methods in Molecular Biology, Vol. 188, Wise, C. ed., Humana Press, Totowa NJ, pp. 315-327.
    10. Bertrand, C.A. (2001). Color by number: imaging large data. Science, 293:1335-1336.

     

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